Core E - Animal Imaging and Tissue Histology
|
Functions
-
In vivo whole animal optical or other molecular imaging or confocal imaging mediated by fiberoptic probes
-
CT, CT/PET, CT/SPECT, MRI animal imaging, ultrasound imaging/ultrasound guided injections.
Animal Imaging Facility
DDC investigators have access to state-of-the-art imaging facilities at the Stanford Center for Innovation in In-Vivo Imaging.
Among the available services available to DDC members:
- in vivo whole animal optical imaging using bioluminescence.
- confocal microscopy using flexible fiber-optic miniprobes
- CT, CT/PET, CT/SPECT, MRI animal imaging, ultrasound imaging/ultrasound guided injections.
http://sci3.stanford.edu/site.html 
Histology Services
Histology Lab is located in Comparative Medicine at Edwards R330. It provides a comprehensive range of histological research techniques that include: standard paraffin processing and embedding methods as well as hand processing for larger specimens; special orientation of tissues; serial sectioning, step sectioning, and sectioning of frozen specimens. Special equipment is available to block tissues in a standardized thickness or orientation to match images from MRI, CT, etc. or for unbiased stereology. In addition to the routine hematoxylin and eosin staining (H&E) of paraffin sections, there are special stains to highlight specific cells, tissue components, bacteria, or other cellular elements and immunoperoxidase and apoptosis assays. Muscle histochemistry and silver stains of nervous system tissues are a specialty. Many histological techniques are sensitive to methods that are used for freezing or fixing the tissues, and results may depend on appropriate specimen preparation. For more information on tissuehistology and service, please directly contact Pauline Chu at 650-723-7235.
Equipment and procedures. Top left - Liver perfusion apparatus. Top right - cannulation of the mouse bile duct. Bottom left and right - rabbit ileal loop model for cholera studies. Assistance was provided to Dr. Gary Schoolnik's laboratory for these studies.
Xenogen bioluminescence imaging systems. Left , IVIS100 (2D) and Middle , IVIS 3D. Right, Bioluminescence imaging of listeriosis in Balb/c mice. (A) Course of lethal intravenous infection over 5 days. Luminescent L. monocytogenes 2C at 4 x 10 4 cfu per mouse (1 LD50), imaged at the indicated days post-infection (bottomnumbers) (B) Oral infection at 5 x 10 9 cfu per mouse, shown on days 1 to 6. The scale bar applies to both images. The images are derived from studies performed in Dr. Contag's laboratory demonstrating extracellular Listeria replication in the murine gallbladder ( Science , 303, 851-853(2004)
PET imaging. Left: microPET R4. Middle and right: transaxial, coronal, and sagittal views of a fused microPET/CT image of melanoma cancer cell model. The cancer cells are marked with HSV1-sr39tk and have metastasized to the lungs and imaged with 18 FHBG.
