Core C - Cell Imaging and Tissue Microarrays
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The Cell Imaging & Tissue Arrays Core offerings are summarized below:
Electron microscopy
All RBIs who wish to use the electron microscopy facility are asked to contact Nafisa Ghori to discuss sample preparation and the application desired. The electron microscopy facility operates as a Service Center, which is partially reimbursed by the DDC for the charges. This is facilitated by the DDC administrator, Linda Jacob.
Confocal microscopy
There are two laser confocal microscopes available to users . One confocal is located in Dr. Falkow's laboratory, and the second identical confocal microscope is available at the VA (managed by Ms. Resurreccion). Both microscopes are capable of simultaneous three-channel sampling at different wavelengths (excluding UV).
Tissue sectioning and immunocychemistry
Evelyn Resurreccion provides assistance and training for the preparation of tissue samples for immunocytochemistry.
Video microscopy
Housed in a room adjacent to the electron microscope suite in Dr. Falkow's laboratory is a video microscope that uses differential interference contrast (Nomarski) optics. This facility features a Nikon Diaphot 200 microscope equipped with a Hamamatsu CCD camera and image processor. In addition, there are two video microscopy units (located at the VA in Dr. Butcher's laboratory) that include an inverted Olympus CK-2 microscope equipped with a high resolution CCD camera, and a highly sensitive silicon-intensified target camera attached to a Zeiss epifluorescence microscope which has the capacity to record real time video imaging.
Tissue Microarrays
This subcore provides unique tissue arrays and the use of existing fetal/adult/GI cancer tissue arrays. It generates TMA's that contain 0.6 mm diameter samples from up to 500 different paraffin embedded specimens. This allows the study to determine the presence and localization of proteins or mRNA species in large numbers of samples in a single experiment.
A more detailed description is provided by Matt van de Rijn at website: http://med.stanford.edu/labs/vanderijn
 Microscope training. Evelyn Resurreccion provides training with the laser confocal microscopes. |
 Confocal Images. Confocal immunofluorescence three-dimensional reconstructions of MDCK monolayers infected with H. pylori for 4 hours ( A ) or 8 hours ( B and C ), and stained with antibodies to ZO-1 (green), E-cadherin (blue), and H. pylori (red) are shown. Yellow areas indicate spatial overlap (colocalization) of the red-stained bacteria with the green ZO-1 signal. Arrows show ZO-1 recruited to extrajunctional sites of bacterial attachment in MDCK cells. Scale bar, 10 µm in (A) and 5 µm in (B) and (C). Science 300: p1430 (2003). Performed by DDC RBIs Manuel Amieva, Stanley Falkow, and W. James Nelson. |
